Formylation of tetrahydrofolate by formyl phosphate
نویسندگان
چکیده
منابع مشابه
Arabidopsis 10-formyl tetrahydrofolate deformylases are essential for photorespiration.
In prokaryotes, PurU (10-formyl tetrahydrofolate [THF] deformylase) metabolizes 10-formyl THF to formate and THF for purine and Gly biosyntheses. The Arabidopsis thaliana genome contains two putative purU genes, At4g17360 and At5g47435. Knocking out these genes simultaneously results in plants that are smaller and paler than the wild type. These double knockout (dKO) mutant plants show a 70-fol...
متن کاملEnzymatic Synthesis of Acetyl Phosphate and Formyl Phosphate by Carbamyl Phosphate Synthetase.
The utilization of rarbamyl phosphate and acetyl phosphate for synthetic pruposes has been studied in thii laboratory (l-4). Frog liver carbamyl phosphate synthetaee catalyzes the synthesis of adenosine triphosphate from acetyl phosphate and adenosine diphosphate (4); it has also been briefly reported that the enzyme could synthesize ATP from formylP and ADP (5). The reverse reactions, the synt...
متن کاملIntracellular metabolism of 5-formyl tetrahydrofolate in human breast and colon cell lines.
This report describes the intracellular metabolism of 5-formyltetrahydrofolate into the various one-carbon substituted folate and polyglutamate pools in a human breast (MCF-7) and colon (HCT 116) carcinoma cell line. Metabolism into the one-carbon substituted pools was found to be time and dose dependent over a concentration range up to 50 microM. A 3-fold increase in total intracellular folate...
متن کاملIi. Enzymatic Synthesis of Formyl Phosphate and Formyl Coenzyme a in Clostridi Um Cylindrosporum
It was incidentally observed in this laboratory that crystalline preparations of formyltetrahydrofolate synthetase from CEostridium cylindrosporum catalyze the activation of formate by adenosine triphosphate in the absence of tetrahydrofolate. Efforts to determine whether the tetrahydrofolate-independent reaction is catalyzed by formyltetrahydrofolate synthetase led eventually to the discovery ...
متن کاملThe enzymes of the 10-formyl-tetrahydrofolate synthetic pathway are found exclusively in the cytosol of the trypanosomatid parasite Leishmania major.
In most organisms 10-formyl-tetrahydrofolate (10-CHO-THF) participates in the synthesis of purines in the cytosol and formylation of mitochondrial initiator methionyl-tRNA(Met). Here we studied 10-CHO-THF biosynthesis in the protozoan parasite Leishmania major, a purine auxotroph. Two distinct synthetic enzymes are known, a bifunctional methylene-tetrahydrofolate dehydrogenase/cyclohydrolase (D...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1990
ISSN: 0014-5793
DOI: 10.1016/0014-5793(90)80231-7